Deciphering the Intracellular Fate and Efficiency of mRNA Delivery. This project aims to improve the effectiveness of mRNA delivered using Lipid Nanoparticles (LNP). There is significant potential to

Description

Deciphering the Intracellular Fate and Efficiency of mRNA Delivery. This project aims to improve the effectiveness of mRNA delivered using Lipid Nanoparticles (LNP). There is significant potential to improve mRNA potency through a deeper understanding of LNP interactions with cells. Our cutting edge approach combines innovative use of long read single cell sequencing with state-of-the-art nanoparticle targeting technology. The outcomes of the project will be: 1) quantifying intact and active mRNA delivered to cells 2) locating where mRNA is degraded 3) assessing cell behaviour after delivery of LNP/mRNA 4) engineering receptor-targeted LNPs that guide delivery of mRNA to where it is required This work will advance LNP delivery technology by maximising RNA expression and minimising off target effects.. Scheme: Discovery Projects. Field: 3106 - Industrial Biotechnology. Lead: A/Prof Angus Johnston